Composite

Part:BBa_K3221010:Design

Designed by: Qihan Jin   Group: iGEM19_SCU-China   (2019-10-05)


pTEF1 - CNS3 - tCYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3749
    Illegal SpeI site found at 2410
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2438
    Illegal SpeI site found at 2410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2882
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3749
    Illegal SpeI site found at 2410
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3749
    Illegal SpeI site found at 2410
    Illegal AgeI site found at 3812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use double enzymes restriction of HindIII and XbaI before adapter ligation.


Source

tCYC1 is on pYES2 initially. We get CNS3 and pTEF1 by gene synthesis.

References